The Light Microscopy Unit and Imaging Unit serves researchers from a range of disciplines, spanning life sciences, physics, chemistry and engineering.
The Unit’s main equipment comprises:
- 3 confocals, two of which are inverted and dedicated to live imaging, and one with a spectral detector and multi-photon laser system.
- 2 fluorescent inverted microscopes
- A pair of fluorescent binoculars
- A High-content-high-throughput imaging system
- Dedicated image analysis and processing software
The Unit provides a full range of services and support, from designing the experiment to data analysis with a full range of software range including:Imaris, Image J/FIJI, ZEN, AxioVision, and InCell Investigator.
- Fast image acquisition for live cell imaging and time-lapse studies
- Z-Stacks for localization of cells in living tissues and 3-D reconstruction
- High-resolution localization of sub-cellular compartments and quantity co-localization
- Spectral imaging and detection
- Fluorescence resonance energy transfer (FRET)
- Multi-channel fluorescence imaging
- Quantitative co-localization
- Fluorescence recovery after photo-bleaching (FRAP)
- Multi-photon imaging
- Automated slide imaging
- Cell-based assays and detection, such as: compound screening, phenotypic profiling, RNAi screening, whole organism imaging, cell lineage studies, cell cycle studies, cell migration, organelle and protein trafficking, morphology analysis, DNA content analysis, and apoptosis/cell viability.